TreeBASE Search-Trees for study 22970

@ARTICLE{TreeBASE2Ref28606, author = {Elizabet D'hooge and Pierre Becker and Dirk Stubbe and Anne-C?cile Normand and Renaud Piarroux and Marijke Hendrickx}, title = {Black aspergilli: a remaining challenge in fungal taxonomy?}, year = {2018}, keywords = {phylogeny, MALDI-TOF MS, antifungal susceptibility, Aspergillus sect. Nigri}, doi = {}, url = {http://}, pmid = {}, journal = {Medical Mycology}, volume = {}, number = {}, pages = {}, abstract = {Aspergillus section Nigri is a taxonomically difficult, but medically and economically important group. In this study, an update of the taxonomy of A. section Nigri strains within the BCCM/IHEM collection has been conducted. The identification accuracy of MALDI-TOF mass spectrometry (MALDI-TOF MS) was tested and the antifungal susceptibilities of clinical isolates were evaluated. A total of 175 strains were molecularly analyzed. Three regions were amplified (ITS, benA and caM) and a multilocus phylogeny of the combined loci was created by using maximum likelihood analysis. The in-house MALDI-TOF MS reference database was extended and an identification dataset of 135 strains was run against a reference dataset. Antifungal susceptibility was tested for voriconazole, itraconazole and amphotericin B, using the EUCAST method. Phylogenetic analysis revealed 18 species in our dataset. MALDI-TOF MS was able to distinguish between A. brasiliensis, A. brunneoviolaceus, A. neoniger, A. niger, A. tubingensis, A. welwitschiae of A. sect. Nigri. In the routine clinical lab, isolates of A. sect. Nigri are often identified as A. niger. However, in the clinical isolates of our dataset, A. tubingensis (n=35) and A. welwitschiae (n=24) are more common than A. niger (n=9). Decreased antifungal susceptibility to azoles was observed in clinical isolates of the /tubingensis clade. This emphasizes the importance of identification up to species level or at least up to clade level in the clinical lab. Our results indicate that MALDI-TOF MS can be a powerful tool to replace classical morphology.} }