TreeBASE Search-Trees for study 23684

@ARTICLE{TreeBASE2Ref29094, author = {Yahui Xuan and Yue Wu and Peng Li and Ruiling Liu and Yiwei Luo and Jianglian Yuan and Zhonghuai Xiang and Ningjia He}, title = {Classification and evolution of Morus based on phylogenetic analyses of ITS and cpDNA sequences}, year = {2018}, keywords = {Morus; Internal transcribed spacer; pseudogenes; concerted evolution; phylogenetic analysis}, doi = {}, url = {http://}, pmid = {}, journal = {BMC Evolutionary Biology}, volume = {}, number = {}, pages = {}, abstract = {Background: The genus Morus (Moraceae) is widely distributed in the world. The classification of Morus is still undetermined, with the characteristics of low resolution in molecular classification and incongruence between molecular and morphological classification. Internal transcribed spacer (ITS) regions (ITS1, 5.8S, and ITS2) are among the most widely used molecular markers in phylogenetic analyses of angiosperms. As one symbol of incomplete concerted evolution of ITS sequences, ITS pseudogenes can act as good supplement in the phylogenetic analyses of ITS sequences. Results: Deep sequencing of ITS sequences in 33 mulberry materials were conducted here. We found ITS pseudogenes in 11 mulberry resources. In the phylogenetic tree constructed from ITS sequences, clade B was separated into short-type sequence clades (clade 1 and clade 2), a long-type sequence clade (clade 3), pseudogroup 1, and pseudogroup 2. cpDNA phylogenetic tree also separated clade B into two clades Conclusions: Two molecular species were separated in clade B. The existence of three connection patterns and incongruent distribution patterns between the cpDNA and ITS sequence phylogenetic tree suggested that the ITS pseudogene sequences connect with genetic information from the female progenitor. Hybridization has played important roles in the evolution of mulberry, and has resulted in low resolution in the classification of ITS sequences. An evolutionary pattern illustrating the evolution history of mulberry is proposed. These findings have significance for the conservation of local mulberry resources.} }