TreeBASE Search-Trees for study 25352

@ARTICLE{TreeBASE2Ref30177, author = {Yen-Ling Lin and Su-Chiung Fang and Chin-Lin Chung and Ming-Hui Chen and Chun-Han Chen}, title = {SUMO Protease SMT7 Modulates Ribosomal Protein L30 and Regulates Cell-size Checkpoint Function}, year = {2020}, keywords = {Chlamydomonas reinhardtii, SUMO protease, cell cycle, cell size, ribosomal protein}, doi = {10.1105/tpc.19.00301}, url = {http://purl.org/phylo/treebase/phylows/study/TB2:S25352}, pmid = {}, journal = {The Plant Cell}, volume = {}, number = {}, pages = {}, abstract = { Proliferating cells actively coordinate growth and cell division to ensure cell-size homeostasis; however, the underlying mechanism through which size is controlled is poorly understood. Defect in a SUMO protease protein, SMT7, has been shown to reduce cell division number and increase cell size of the small-size mutant mating type locus 3-4 (mat3-4), which contains a defective Chlamydomonas retinoblastoma tumor suppressor-related protein. Here we describe development of an in vitro SUMOylation system using Chlamydomonas components and use it to provide evidence that SMT7 is a bona fide SUMO protease. We further demonstrate that the SUMO protease activity is required for supernumerous mitotic divisions of the mat3-4 cells. In addition, we identified ribosomal protein L30 (RPL30) as a prime SMT7 target and demonstrated that its SUMOylation is an important modulator of cell division in mat3-4 cells. Loss of SMT7 caused elevated SUMOylated RPL30 levels. Importantly, overexpression of the translational fusion version of RPL30-SUMO4, which mimics elevation of the SUMOylated RPL30 protein in mat3-4, caused a decrease in mitotic division and recapitulated the size-increasing phenotype of the smt7-1 mat3-4 cells. In summary, our study reveals a novel mechanism through which a SUMO protease regulates cell division in the mat3-4 mutant of Chlamydomonas and provides yet another important example of the role that protein SUMOylation can play in regulating key cellular processes, including cell division.} }