@ARTICLE{TreeBASE2Ref17532, author = {Bernard Slippers and Pedro W. Crous and Sandra Denman and Teresa A. Coutinho and Brenda D Wingfield and Michael J Wingfield}, title = {Combined multiple gene genealogies and phenotype characters differentiate several species previously identified as Botryosphaeria dothidea.}, year = {2003}, keywords = {}, doi = {}, url = {}, pmid = {}, journal = {Mycologia}, volume = {}, number = {}, pages = {}, abstract = {Botryosphaeria dothidea is one of the most commonly reported species in a genus of important pathogens of woody plants. This taxon is generally accepted to represent a species complex, and hence its identity remains unclear. Previous studies have either treated B. dothidea as the valid name for B. ribis and B. berengeriana, or argued for them to be separate entities. To add to the confusion, no ex-type cultures are available for either B. dothidea or B. ribis. The aim of the present study, therefore, was to recollect and characterize these fungi, and designate a set of reference cultures that can be used in future studies. To this end morphological, cultural, and multi-allelic DNA sequence data sets from the rDNA (ITS 1, 5.8S, and ITS 2), b-tubulin and EF1-a genes were used to fully characterize these species. Botryosphaeria dothidea was found to be distinct from B. ribis, while B. berengeriana was retained as synonym of the former name. Furthermore, Fusicoccum aesculi is accepted as anamorph of B. dothidea, while the anamorph of B. ribis is newly described as F. ribis sp. nov. Botryosphaeria ribis could be distinguished from B. parva based on b-tubulin and EF1-a sequence data. A combined phylogeny of the three gene regions used in this study also showed that the genus Botryosphaeria represents two distinct phylogenetic assemblages that correspond to species with Diplodia and Fusicoccum anamorphs.} }

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