@ARTICLE{TreeBASE2Ref16802, author = {Henrietta Myburg and Marieka Gryzenhout and Brenda D Wingfield and Michael J Wingfield}, title = {Conspecificity of Endothia eugeniae and Cryphonectria cubensis: A re-evaluation based on morphology and DNA sequence data.}, year = {2003}, keywords = {}, doi = {}, url = {}, pmid = {}, journal = {Mycoscience}, volume = {44}, number = {3}, pages = {}, abstract = {Cryphonectria cubensis and Endothia eugeniae are fungal pathogens of Eucalyptus and clove that were reduced to synonymy based on the results of cross-inoculation studies, isozyme analysis, cultural studies and morphology. A previous phylogenetic study on Cryphonectria, based on sequence variation in the ITS regions of the ribosomal RNA operon, also supported the conspecificity of C. cubensis and E. eugeniae, but was based on only one E. eugeniae isolate. New collections from clove in Brazil and Indonesia have become available, providing the opportunity to reconsider the conspecificity of C. cubensis and E. eugeniae. The occurrence of C. cubensis on clove was confirmed based on morphological comparisons and phylogenetic analyses of ribosomal DNA and b-tubulin gene sequence data. In addition to C. cubensis, other fungi morphologically similar to Cryphonectria species based on their orange stromata, were also present on some clove specimens. Furthermore, a smaller number of isolates, for which no herbarium material exists, grouped separately, outside both the C. cubensis and Cryphonectria clade. The presence of more than one closely related fungus on clove, raises questions relating to the legitimacy of the synonymy of E. eugeniae and C. cubensis, but the presence of C. cubensis on the type specimen of E. eugeniae supports it. We thus retain the synonymy of the two fungi but provide evidence that other fungi, closely related to Cryphonectria but not C. cubensis, are present on clove.} }

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