@ARTICLE{TreeBASE2Ref15428,
author = {Katerina Fliegerov? and B. Hodrov? and Kerstin Voigt},
title = {Classical and Molecular Approaches as a Powerful Tool for the Characterization of Rumen Polycentric Fungi.},
year = {2004},
keywords = {},
doi = {},
url = {},
pmid = {},
journal = {Folia Microbiologica (Praha)},
volume = {49},
number = {2},
pages = {157--164},
abstract = {The ribosomal ITS 1 and ITS 2 fragments from 8 isolates of polycentric rumen anaerobic fungi were PCR-amplified and sequenced; the sequences obtained were aligned with published data and phylogenetic analyses were performed. The analysis of ITS 1 fragment clearly differentiated between the two polycentric genera Orpinomyces and Anaeromyces and these classification is supported by morphological observation. A multi-order phylogram based on ITS2 sequences proved that anaerobic rumen fungi are separated from aerobic chytrids, which form a well-supported monophylum with highest possible bootstrap proportion values of 100 % . Sequence analysis of ITS regions is a powerful tool for the classification of anaerobic fungi, however morphological description of strains is still necessary because some genera of rumen fungi display a high genetic heterogeneity.}
}
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Citation title: "Classical and Molecular Approaches as a Powerful Tool for the Characterization of Rumen Polycentric Fungi.".
