TreeBASE Search-Citation for study 1074

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Citation for Study 1074

Citation title: "Classical and Molecular Approaches as a Powerful Tool for the Characterization of Rumen Polycentric Fungi.".

This study was previously identified under the legacy study ID S976 (Status: Published).

Citation

Fliegerov? K., Hodrov? B., & Voigt K. 2004. Classical and Molecular Approaches as a Powerful Tool for the Characterization of Rumen Polycentric Fungi. Folia Microbiologica (Praha), 49(2): 157-164.

Authors

Fliegerov? K.
Hodrov? B.
Voigt K.

Abstract

The ribosomal ITS 1 and ITS 2 fragments from 8 isolates of polycentric rumen anaerobic fungi were PCR-amplified and sequenced; the sequences obtained were aligned with published data and phylogenetic analyses were performed. The analysis of ITS 1 fragment clearly differentiated between the two polycentric genera Orpinomyces and Anaeromyces and these classification is supported by morphological observation. A multi-order phylogram based on ITS2 sequences proved that anaerobic rumen fungi are separated from aerobic chytrids, which form a well-supported monophylum with highest possible bootstrap proportion values of 100 % . Sequence analysis of ITS regions is a powerful tool for the classification of anaerobic fungi, however morphological description of strains is still necessary because some genera of rumen fungi display a high genetic heterogeneity.

About this resource

Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S1074
Other versions: Nexus NeXML

Show BibTeX reference

		@ARTICLE{TreeBASE2Ref15428,
	 author = {Katerina  Fliegerov? and B.  Hodrov? and Kerstin  Voigt},
	 title = {Classical and Molecular Approaches as a Powerful Tool for the Characterization of Rumen Polycentric Fungi.},
	 year = {2004},
	 keywords = {},
	 doi = {},
	 url = {},
	 pmid = {},
	 journal = {Folia Microbiologica (Praha)},
	 volume = {49},
	 number = {2},
	 pages = {157--164},
	 abstract = {The ribosomal ITS 1 and ITS 2 fragments from 8 isolates of polycentric rumen anaerobic fungi were PCR-amplified and sequenced; the sequences obtained were aligned with published data and phylogenetic analyses were performed. The analysis of ITS 1 fragment clearly differentiated between the two polycentric genera Orpinomyces and Anaeromyces and these classification is supported by morphological observation. A multi-order phylogram based on ITS2 sequences proved that anaerobic rumen fungi are separated from aerobic chytrids, which form a well-supported monophylum with highest possible bootstrap proportion values of 100 % . Sequence analysis of ITS regions is a powerful tool for the classification of anaerobic fungi, however morphological description of strains is still necessary because some genera of rumen fungi display a high genetic heterogeneity.}
}

Show RIS reference

		TY  - JOUR
ID  - 15428
AU  - Fliegerov?,Katerina 
AU  - Hodrov?,B. 
AU  - Voigt,Kerstin 
T1  - Classical and Molecular Approaches as a Powerful Tool for the Characterization of Rumen Polycentric Fungi.
PY  - 2004
UR  - 
N2  - The ribosomal ITS 1 and ITS 2 fragments from 8 isolates of polycentric rumen anaerobic fungi were PCR-amplified and sequenced; the sequences obtained were aligned with published data and phylogenetic analyses were performed. The analysis of ITS 1 fragment clearly differentiated between the two polycentric genera Orpinomyces and Anaeromyces and these classification is supported by morphological observation. A multi-order phylogram based on ITS2 sequences proved that anaerobic rumen fungi are separated from aerobic chytrids, which form a well-supported monophylum with highest possible bootstrap proportion values of 100 % . Sequence analysis of ITS regions is a powerful tool for the classification of anaerobic fungi, however morphological description of strains is still necessary because some genera of rumen fungi display a high genetic heterogeneity.
L3  - 
JF  - Folia Microbiologica (Praha)
VL  - 49
IS  - 2
SP  - 157
EP  - 164
ER  -