TreeBASE Search-Matrices for study 13647

@ARTICLE{TreeBASE2Ref21546, author = {Huzefa A Raja and Kazuaki Tanaka and Kazuyuki Hirayama and Akira Hashimoto and Nicholas Oberlies and Mario Figueroa and Andrew N. Miller and Steven E Zelski and Carol A. Shearer}, title = {Freshwater Ascomycetes: Minutisphaera (Dothideomycetes) revisited, including one new species from Japan}, year = {2013}, keywords = {}, doi = {}, url = {http://}, pmid = {}, journal = {Mycologia}, volume = {}, number = {}, pages = {}, abstract = {During ongoing investigations of freshwater ascomycetes we found interesting taxa from Aomori, (Japan) as well as North Carolina (USA), which were morphologically similar to Minutisphaera, a recently described freshwater fungus in the Dothideomycetes. These taxa showed dark hair-like structures around the ostiolar region, obovoid to obclavate bitunicate asci, and one to three septate hyaline to brown ascospores with a sheath (in material from Japan), and with sheath and appendages (in material from the USA). The ascomata of these taxa, however, were more apothecioid than those of the type species of the genus, which were perithecoid. In addition, two collections of Minutisphaera-like fungi from the USA, which were morphologically quite similar, showed variation in ascospore size. In order to assess the phylogenetic affinities of these taxa with the type species Minutisphaera fimbriatispora, we sequenced the 18S and 28S nrDNA. We also sequenced the Internal Transcribed Spacer regions, 1 and 2 including the 5.8S nrDNA to assess interspecific and intraspecific variation of the newly collected strains with M. fimbriatispora. Additionally, we examined the secondary metabolite profiles of two isolates from the USA. Based on Maximum Likelihood and Bayesian analyses of sequences (on combined 18S and 28S, and separate ITS), as well as examination of morphology, we describe and illustrate a new species, Minutisphaera japonica. One collection from North Carolina comprised of M. fimbriatispora, while two other collections were Minutisphaera-like fungi that showed a number of similar diagnostic morphological characters, but differed only slightly in their ascospore size and cultural characteristics. Phylogeny inferred from the ITS region suggested that these two North Carolina collections may be putatively novel and perhaps cryptic species within Minutisphaera, which requires additional study. Organic extracts of Minutisphaera from the USA, M. fimbriatispora (G155-1), and Minutisphaera-like taxon (G156-1) both revealed the presence of palmitic acid and (E)-hexadec-9-en-1-ol as major chemical constituents. We discuss the placement of these taxa within the Dothideomycetes. In order to accommodate M. japonica within Minutisphaera on the basis of morphology, we emend the description of the genus Minutisphaera. } }