@ARTICLE{TreeBASE2Ref17364, author = {Scott R. Santos and Derek J. Taylor and Mary Alice Coffroth}, title = {Genetic comparisons of freshly isolated vs. cultured symbiotic dinoflagellates: Implications for extrapolation to the intact symbiosis.}, year = {2001}, keywords = {culture; internal transcribed spacer; microsatellites; small subunit rDNA genes; Symbiodinium; symbiosis; symbiotic dinoflagellates; zooxanthellae}, doi = {10.1046/j.1529-8817.2001.00194.x}, url = {}, pmid = {}, journal = {Journal of Phycology}, volume = {37}, number = {5}, pages = {900--912}, abstract = {Zooxanthellae, algal symbionts in divergent marine invertebrate hosts, are a genetically heterogeneous group. All species descriptions and most physiological and infection studies of zooxanthellae have been conducted using cultured material, yet few studies to date have attempted to quantify the representation of cultures isolated from cnidarians to the in hospite zooxanthella populations from which they were established. Restriction Fragment Length Polymorphisms (RFLPs) of 18S-rDNA, Internal Transcribed Spacer (ITS) sequence data and microsatellites analyses were conducted to assess the relatedness between cultured zooxanthellae and the in hospite population from which they were isolated. RFLP data demonstrated cultures may represent either the numerically dominant symbiont or ones present in lower number. ITS sequences from zooxanthella cultures are disconcordant with ITS sequence identified from the in hospite zooxanthellae and microsatellites present in in hospite zooxanthella populations were absent from the corresponding cultures. Lastly, reexamination of the literature reveals examples of zooxanthella cultures being nonrepresentative of in hospite populations. These data suggest that, in most cases, cultures are a subset of the original in hospite population. Factors such as failing to homogenize bulk cultures prior to transfer, growth medium employed and the picking of single motile cells are thought to contribute to many zooxanthella cultures being nonrepresentative.} }

• Go to search
• Citation
• Taxa
• Matrices
• Trees
• Analyses
• 

CiteULike

Delicious

Connotea