TreeBASE Search-Matrices for study 973

@ARTICLE{TreeBASE2Ref15344, author = {Esra Einax and Kerstin Voigt}, title = {Oligonucleotide primers for the universal amplification of beta-tubulin genes facilitate analyses among the regnum FUNGI.}, year = {2003}, keywords = {}, doi = {}, url = {}, pmid = {}, journal = {Organisms Diversity & Evolution}, volume = {3}, number = {3}, pages = {185--194}, abstract = {Among genes coding for proteins with basic structural functions in all eukaryotes, the highly conserved and functionally essential gene for beta-tubulin is receiving increasing attention in the reconstruction of phylogenies within a broad organismic range. We therefore constructed a set of twelve universally applicable primers that allow reliable amplification of beta-tubulin genes among all major eukaryotic kingdoms including fungi (Eumycota), animals (Metazoa) and green plants (Viridiplantae). For primer design, the amino acid sequences of 35 beta-tubulin genes from Ascomycota, Basidiomycota, Chytridiomycota, Zygomycota, Metazoa, Oophyta and Viridiplantae were aligned and used for the definition of four well-conserved regions. These are suitable priming sites in PCR amplification experiments. Out of these amino acid regions twelve primers were designed, which initiate the amplification of beta-tubulin genes from a wide range of eukaryotic organisms with special emphasis on fungi. In four pair-wise primer applications gene fragments of up to 1,500 bp in size could be isolated, which comprise nearly complete beta-tubulin genes from twelve representative species of the Eumycota. The sequences of 7 beta-tubulin fragments were obtained from Allomyces moniliformis, A. neomoniliformis, Blastocladiella britannica, Chytridium confervae, Mortierella isabellina and Trametes versicolor. Reliable amplification of beta-tubulin over a broad spectrum of organisms provides a strong basis for the establishment of both deep level phylogenies and studies of complex species groups based on beta-tubulin gene trees.} }