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Citation for Study 973

Citation title: "Oligonucleotide primers for the universal amplification of beta-tubulin genes facilitate analyses among the regnum FUNGI.".

This study was previously identified under the legacy study ID S857 (Status: Published).

Citation

Einax E., & Voigt K. 2003. Oligonucleotide primers for the universal amplification of beta-tubulin genes facilitate analyses among the regnum FUNGI. Organisms Diversity & Evolution, 3(3): 185-194.

Authors

• Einax E.
• Voigt K.

Abstract

Among genes coding for proteins with basic structural functions in all eukaryotes, the highly conserved and functionally essential gene for beta-tubulin is receiving increasing attention in the reconstruction of phylogenies within a broad organismic range. We therefore constructed a set of twelve universally applicable primers that allow reliable amplification of beta-tubulin genes among all major eukaryotic kingdoms including fungi (Eumycota), animals (Metazoa) and green plants (Viridiplantae). For primer design, the amino acid sequences of 35 beta-tubulin genes from Ascomycota, Basidiomycota, Chytridiomycota, Zygomycota, Metazoa, Oophyta and Viridiplantae were aligned and used for the definition of four well-conserved regions. These are suitable priming sites in PCR amplification experiments. Out of these amino acid regions twelve primers were designed, which initiate the amplification of beta-tubulin genes from a wide range of eukaryotic organisms with special emphasis on fungi. In four pair-wise primer applications gene fragments of up to 1,500 bp in size could be isolated, which comprise nearly complete beta-tubulin genes from twelve representative species of the Eumycota. The sequences of 7 beta-tubulin fragments were obtained from Allomyces moniliformis, A. neomoniliformis, Blastocladiella britannica, Chytridium confervae, Mortierella isabellina and Trametes versicolor. Reliable amplification of beta-tubulin over a broad spectrum of organisms provides a strong basis for the establishment of both deep level phylogenies and studies of complex species groups based on beta-tubulin gene trees.

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S973
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref15344,
  	 author = {Esra  Einax and Kerstin  Voigt},
  	 title = {Oligonucleotide primers for the universal amplification of beta-tubulin genes facilitate analyses among the regnum FUNGI.},
  	 year = {2003},
  	 keywords = {},
  	 doi = {},
  	 url = {},
  	 pmid = {},
  	 journal = {Organisms Diversity & Evolution},
  	 volume = {3},
  	 number = {3},
  	 pages = {185--194},
  	 abstract = {Among genes coding for proteins with basic structural functions in all eukaryotes, the highly conserved and functionally essential gene for beta-tubulin is receiving increasing attention in the reconstruction of phylogenies within a broad organismic range. We therefore constructed a set of twelve universally applicable primers that allow reliable amplification of beta-tubulin genes among all major eukaryotic kingdoms including fungi (Eumycota), animals (Metazoa) and green plants (Viridiplantae). For primer design, the amino acid sequences of 35 beta-tubulin genes from Ascomycota, Basidiomycota, Chytridiomycota, Zygomycota, Metazoa, Oophyta and Viridiplantae were aligned and used for the definition of four well-conserved regions. These are suitable priming sites in PCR amplification experiments. Out of these amino acid regions twelve primers were designed, which initiate the amplification of beta-tubulin genes from a wide range of eukaryotic organisms with special emphasis on fungi. In four pair-wise primer applications gene fragments of up to 1,500 bp in size could be isolated, which comprise nearly complete beta-tubulin genes from twelve representative species of the Eumycota. The sequences of 7 beta-tubulin fragments were obtained from Allomyces moniliformis, A. neomoniliformis, Blastocladiella britannica, Chytridium confervae, Mortierella isabellina and Trametes versicolor. Reliable amplification of beta-tubulin over a broad spectrum of organisms provides a strong basis for the establishment of both deep level phylogenies and studies of complex species groups based on beta-tubulin gene trees.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 15344
  AU  - Einax,Esra 
  AU  - Voigt,Kerstin 
  T1  - Oligonucleotide primers for the universal amplification of beta-tubulin genes facilitate analyses among the regnum FUNGI.
  PY  - 2003
  UR  - 
  N2  - Among genes coding for proteins with basic structural functions in all eukaryotes, the highly conserved and functionally essential gene for beta-tubulin is receiving increasing attention in the reconstruction of phylogenies within a broad organismic range. We therefore constructed a set of twelve universally applicable primers that allow reliable amplification of beta-tubulin genes among all major eukaryotic kingdoms including fungi (Eumycota), animals (Metazoa) and green plants (Viridiplantae). For primer design, the amino acid sequences of 35 beta-tubulin genes from Ascomycota, Basidiomycota, Chytridiomycota, Zygomycota, Metazoa, Oophyta and Viridiplantae were aligned and used for the definition of four well-conserved regions. These are suitable priming sites in PCR amplification experiments. Out of these amino acid regions twelve primers were designed, which initiate the amplification of beta-tubulin genes from a wide range of eukaryotic organisms with special emphasis on fungi. In four pair-wise primer applications gene fragments of up to 1,500 bp in size could be isolated, which comprise nearly complete beta-tubulin genes from twelve representative species of the Eumycota. The sequences of 7 beta-tubulin fragments were obtained from Allomyces moniliformis, A. neomoniliformis, Blastocladiella britannica, Chytridium confervae, Mortierella isabellina and Trametes versicolor. Reliable amplification of beta-tubulin over a broad spectrum of organisms provides a strong basis for the establishment of both deep level phylogenies and studies of complex species groups based on beta-tubulin gene trees.
  L3  - 
  JF  - Organisms Diversity & Evolution
  VL  - 3
  IS  - 3
  SP  - 185
  EP  - 194
  ER  -