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Citation for Study 19741

Citation title: "Genome-wide analysis of TPX2 family proteins in Eucalyptus grandis".

Study name: "Genome-wide analysis of TPX2 family proteins in Eucalyptus grandis".

This study is part of submission 19741 (Status: Published).

Citation

Du P., Kumar M., Yao Y., Xie Q., Wang J., Zhang B., Gan S., Wang Y., & Wu A. 2016. Genome-wide analysis of TPX2 family proteins in Eucalyptus grandis. BMC genomics, .

Authors

• Du P.
• Kumar M. (submitter) 1612751710
• Yao Y.
• Xie Q.
• Wang J.
• Zhang B.
• Gan S.
• Wang Y.
• Wu A.

Abstract

Background: The targeting protein for Xklp2 (TPX2) family is a microtubule-associated protein (MAP) family. All members of the family contain the conserved TPX2 motif, which can interact with microtubules, regulate microtubule dynamics or assist different microtubule roles, for example, maintain the cell morphology or regulate cell growth and development. However, the role of none of the members of this family has been studied in the model tree species Eucalyptus to date. Here, we report the identification of the members of the TPX2 family in Eucalyptus grandis (Eg) and analyse the expression patterns and functions of these genes. Results: In the present study, a comprehensive analysis of the plant TPX2 family proteins was performed. Phylogenetic analyses indicated that these genes can be further classified into 6 distinct subfamilies. A genome-wide survey identified 12 members of the TPX2 family in the sequenced genome of Eucalyptus grandis. The basic genetic properties of the TPX2 family in Eucalyptus were analysed. Our results suggest that the TPX2 family proteins in different sub-groups are relatively conserved but there are important differences between groups. Quantitative real-time PCR (qRT-PCR) was performed to confirm the expression levels of these genes in different tissues. The results showed that in the whole plant, the levels of EgWDL5 transcript are the highest, followed by those of EgWDL4. Compared with other tissues, the level of EgMAP20 transcription is the highest in root. Over-expression of EgMAP20 in Arabidopsis resulted in organ twisting. The cotyledon petioles showed left-handed twisting while the hypocotyl epidermal cells produced right-handed helical twisting. Finally, EgMAP20, EgWDL3 and EgWDL3L were all able to decorate microtubules. Conclusions: Plant TPX2 family proteins were systematically analysed using bioinformatics methods. There are 12 TPX2 family proteins in Eucalyptus. We preliminarily characterized the functions of several members of the TPX2 family. We found that the gene products are localized to the microtubule cytoskeleton. Our results lay the foundation for future efforts to reveal the biological significance of TPX2 family proteins in Eucalyptus.

Keywords

Eucalyptus, TPX2 family proteins, Phylogenetic Analysis, Expression profiling, Subcellular localization, Organ twisting

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S19741
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref26227,
  	 author = {Pingzhou  Du and Manoj  Kumar and Yuan  Yao and Qiaoli  Xie and Jinyan  Wang and Baolong  Zhang and Siming  Gan and Yuqi  Wang and Ai-Min  Wu},
  	 title = {Genome-wide analysis of TPX2 family proteins in Eucalyptus grandis},
  	 year = {2016},
  	 keywords = {Eucalyptus, TPX2 family proteins, Phylogenetic Analysis, Expression profiling, Subcellular localization, Organ twisting},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {BMC genomics},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Background: The targeting protein for Xklp2 (TPX2) family is a microtubule-associated protein (MAP) family. All members of the family contain the conserved TPX2 motif, which can interact with microtubules, regulate microtubule dynamics or assist different microtubule roles, for example, maintain the cell morphology or regulate cell growth and development. However, the role of none of the members of this family has been studied in the model tree species Eucalyptus to date. Here, we report the identification of the members of the TPX2 family in Eucalyptus grandis (Eg) and analyse the expression patterns and functions of these genes.
  Results: In the present study, a comprehensive analysis of the plant TPX2 family proteins was performed. Phylogenetic analyses indicated that these genes can be further classified into 6 distinct subfamilies. A genome-wide survey identified 12 members of the TPX2 family in the sequenced genome of Eucalyptus grandis. The basic genetic properties of the TPX2 family in Eucalyptus were analysed. Our results suggest that the TPX2 family proteins in different sub-groups are relatively conserved but there are important differences between groups. Quantitative real-time PCR (qRT-PCR) was performed to confirm the expression levels of these genes in different tissues. The results showed that in the whole plant, the levels of EgWDL5 transcript are the highest, followed by those of EgWDL4. Compared with other tissues, the level of EgMAP20 transcription is the highest in root. Over-expression of EgMAP20 in Arabidopsis resulted in organ twisting. The cotyledon petioles showed left-handed twisting while the hypocotyl epidermal cells produced right-handed helical twisting. Finally, EgMAP20, EgWDL3 and EgWDL3L were all able to decorate microtubules.
  Conclusions: Plant TPX2 family proteins were systematically analysed using bioinformatics methods. There are 12 TPX2 family proteins in Eucalyptus. We preliminarily characterized the functions of several members of the TPX2 family. We found that the gene products are localized to the microtubule cytoskeleton. Our results lay the foundation for future efforts to reveal the biological significance of TPX2 family proteins in Eucalyptus. 
  }
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 26227
  AU  - Du,Pingzhou 
  AU  - Kumar,Manoj 
  AU  - Yao,Yuan 
  AU  - Xie,Qiaoli 
  AU  - Wang,Jinyan 
  AU  - Zhang,Baolong 
  AU  - Gan,Siming 
  AU  - Wang,Yuqi 
  AU  - Wu,Ai-Min 
  T1  - Genome-wide analysis of TPX2 family proteins in Eucalyptus grandis
  PY  - 2016
  KW  - Eucalyptus
  KW  - TPX2 family proteins
  KW  - Phylogenetic Analysis
  KW  - Expression profiling
  KW  - Subcellular localization
  KW  - Organ twisting
  UR  - http://dx.doi.org/
  N2  - Background: The targeting protein for Xklp2 (TPX2) family is a microtubule-associated protein (MAP) family. All members of the family contain the conserved TPX2 motif, which can interact with microtubules, regulate microtubule dynamics or assist different microtubule roles, for example, maintain the cell morphology or regulate cell growth and development. However, the role of none of the members of this family has been studied in the model tree species Eucalyptus to date. Here, we report the identification of the members of the TPX2 family in Eucalyptus grandis (Eg) and analyse the expression patterns and functions of these genes.
  Results: In the present study, a comprehensive analysis of the plant TPX2 family proteins was performed. Phylogenetic analyses indicated that these genes can be further classified into 6 distinct subfamilies. A genome-wide survey identified 12 members of the TPX2 family in the sequenced genome of Eucalyptus grandis. The basic genetic properties of the TPX2 family in Eucalyptus were analysed. Our results suggest that the TPX2 family proteins in different sub-groups are relatively conserved but there are important differences between groups. Quantitative real-time PCR (qRT-PCR) was performed to confirm the expression levels of these genes in different tissues. The results showed that in the whole plant, the levels of EgWDL5 transcript are the highest, followed by those of EgWDL4. Compared with other tissues, the level of EgMAP20 transcription is the highest in root. Over-expression of EgMAP20 in Arabidopsis resulted in organ twisting. The cotyledon petioles showed left-handed twisting while the hypocotyl epidermal cells produced right-handed helical twisting. Finally, EgMAP20, EgWDL3 and EgWDL3L were all able to decorate microtubules.
  Conclusions: Plant TPX2 family proteins were systematically analysed using bioinformatics methods. There are 12 TPX2 family proteins in Eucalyptus. We preliminarily characterized the functions of several members of the TPX2 family. We found that the gene products are localized to the microtubule cytoskeleton. Our results lay the foundation for future efforts to reveal the biological significance of TPX2 family proteins in Eucalyptus. 
  
  L3  - 
  JF  - BMC genomics
  VL  - 
  IS  - 
  ER  -