@ARTICLE{TreeBASE2Ref26227, author = {Pingzhou Du and Manoj Kumar and Yuan Yao and Qiaoli Xie and Jinyan Wang and Baolong Zhang and Siming Gan and Yuqi Wang and Ai-Min Wu}, title = {Genome-wide analysis of TPX2 family proteins in Eucalyptus grandis}, year = {2016}, keywords = {Eucalyptus, TPX2 family proteins, Phylogenetic Analysis, Expression profiling, Subcellular localization, Organ twisting}, doi = {}, url = {http://}, pmid = {}, journal = {BMC genomics}, volume = {}, number = {}, pages = {}, abstract = {Background: The targeting protein for Xklp2 (TPX2) family is a microtubule-associated protein (MAP) family. All members of the family contain the conserved TPX2 motif, which can interact with microtubules, regulate microtubule dynamics or assist different microtubule roles, for example, maintain the cell morphology or regulate cell growth and development. However, the role of none of the members of this family has been studied in the model tree species Eucalyptus to date. Here, we report the identification of the members of the TPX2 family in Eucalyptus grandis (Eg) and analyse the expression patterns and functions of these genes. Results: In the present study, a comprehensive analysis of the plant TPX2 family proteins was performed. Phylogenetic analyses indicated that these genes can be further classified into 6 distinct subfamilies. A genome-wide survey identified 12 members of the TPX2 family in the sequenced genome of Eucalyptus grandis. The basic genetic properties of the TPX2 family in Eucalyptus were analysed. Our results suggest that the TPX2 family proteins in different sub-groups are relatively conserved but there are important differences between groups. Quantitative real-time PCR (qRT-PCR) was performed to confirm the expression levels of these genes in different tissues. The results showed that in the whole plant, the levels of EgWDL5 transcript are the highest, followed by those of EgWDL4. Compared with other tissues, the level of EgMAP20 transcription is the highest in root. Over-expression of EgMAP20 in Arabidopsis resulted in organ twisting. The cotyledon petioles showed left-handed twisting while the hypocotyl epidermal cells produced right-handed helical twisting. Finally, EgMAP20, EgWDL3 and EgWDL3L were all able to decorate microtubules. Conclusions: Plant TPX2 family proteins were systematically analysed using bioinformatics methods. There are 12 TPX2 family proteins in Eucalyptus. We preliminarily characterized the functions of several members of the TPX2 family. We found that the gene products are localized to the microtubule cytoskeleton. Our results lay the foundation for future efforts to reveal the biological significance of TPX2 family proteins in Eucalyptus. } }

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