@ARTICLE{TreeBASE2Ref31270,
author = {Felix LaRoche-Johnston and Rafia Bosan and Benoit Cousineau},
title = {Group II introns generate functional chimeric relaxase enzymes with modified specificities through exon shuffling at both the RNA and DNA level},
year = {2020},
keywords = {Group II introns, Lactococcus lactis, Enterococcus faecalis, Ll.LtrB, Ef.PcfG, conjugation, trans-splicing, bacterial genetics, genetic diversity, molecular evolution.},
doi = {10.1093/molbev/msaa275},
url = {http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947834/pdf/msaa275.pdf},
pmid = {33118013},
journal = {Molecular Biology and Evolution},
volume = {38},
number = {3},
pages = {1075--1089},
abstract = {Group II introns are large self-splicing RNA enzymes with a broad but somewhat irregular phylogenetic distribution. These ancient retromobile elements are the proposed ancestors of approximately half the human genome, including the abundant spliceosomal introns and non-LTR retrotransposons. In contrast to their eukaryotic derivatives, bacterial group II introns have largely been considered as harmful selfish mobile retroelements that parasitize the genome of their host. As a challenge to this view, we recently uncovered a new intergenic trans-splicing pathway that generates an assortment of mRNA chimeras. The ability of group II introns to combine disparate mRNA fragments was proposed to increase the genetic diversity of the bacterial host by shuffling coding sequences. Here we show that the Ll.LtrB and Ef.PcfG group II introns from Lactococcus lactis and Enterococcus faecalis respectively can both use the intergenic trans-splicing pathway to catalyze the formation of chimeric relaxase mRNAs and functional proteins. We demonstrated that some of these compound relaxase enzymes yield gain-of-function phenotypes, being significantly more efficient than their precursor wild-type enzymes at supporting bacterial conjugation. We also found that relaxase enzymes with shuffled functional domains are produced in biologically relevant settings under natural expression levels. Finally, we uncovered examples of lactococcal chimeric relaxase genes with junctions exactly at the intron insertion site. Overall, our work demonstrates that the genetic diversity generated by group II introns, at the RNA level by intergenic trans-splicing and at the DNA level most likely by recombination, can yield new functional enzymes with shuffled exons, which can lead to gain-of-function phenotypes. }
}
Citation for Study 27000
Citation title:
"Group II introns generate functional chimeric relaxase enzymes with modified specificities through exon shuffling at both the RNA and DNA level".
Study name:
"Group II introns generate functional chimeric relaxase enzymes with modified specificities through exon shuffling at both the RNA and DNA level".
This study is part of submission 27000
(Status: Published).
Citation
Laroche-johnston F., Bosan R., & Cousineau B. 2020. Group II introns generate functional chimeric relaxase enzymes with modified specificities through exon shuffling at both the RNA and DNA level. Molecular Biology and Evolution, 38(3): 1075-1089.
Authors
-
Laroche-johnston F.
(submitter)
-
Bosan R.
-
Cousineau B.
Abstract
Group II introns are large self-splicing RNA enzymes with a broad but somewhat irregular phylogenetic distribution. These ancient retromobile elements are the proposed ancestors of approximately half the human genome, including the abundant spliceosomal introns and non-LTR retrotransposons. In contrast to their eukaryotic derivatives, bacterial group II introns have largely been considered as harmful selfish mobile retroelements that parasitize the genome of their host. As a challenge to this view, we recently uncovered a new intergenic trans-splicing pathway that generates an assortment of mRNA chimeras. The ability of group II introns to combine disparate mRNA fragments was proposed to increase the genetic diversity of the bacterial host by shuffling coding sequences. Here we show that the Ll.LtrB and Ef.PcfG group II introns from Lactococcus lactis and Enterococcus faecalis respectively can both use the intergenic trans-splicing pathway to catalyze the formation of chimeric relaxase mRNAs and functional proteins. We demonstrated that some of these compound relaxase enzymes yield gain-of-function phenotypes, being significantly more efficient than their precursor wild-type enzymes at supporting bacterial conjugation. We also found that relaxase enzymes with shuffled functional domains are produced in biologically relevant settings under natural expression levels. Finally, we uncovered examples of lactococcal chimeric relaxase genes with junctions exactly at the intron insertion site. Overall, our work demonstrates that the genetic diversity generated by group II introns, at the RNA level by intergenic trans-splicing and at the DNA level most likely by recombination, can yield new functional enzymes with shuffled exons, which can lead to gain-of-function phenotypes.
Keywords
Group II introns, Lactococcus lactis, Enterococcus faecalis, Ll.LtrB, Ef.PcfG, conjugation, trans-splicing, bacterial genetics, genetic diversity, molecular evolution.
External links
About this resource
- Canonical resource URI:
http://purl.org/phylo/treebase/phylows/study/TB2:S27000
- Other versions:
Nexus
NeXML
- Show BibTeX reference
@ARTICLE{TreeBASE2Ref31270,
author = {Felix LaRoche-Johnston and Rafia Bosan and Benoit Cousineau},
title = {Group II introns generate functional chimeric relaxase enzymes with modified specificities through exon shuffling at both the RNA and DNA level},
year = {2020},
keywords = {Group II introns, Lactococcus lactis, Enterococcus faecalis, Ll.LtrB, Ef.PcfG, conjugation, trans-splicing, bacterial genetics, genetic diversity, molecular evolution.},
doi = {10.1093/molbev/msaa275},
url = {http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947834/pdf/msaa275.pdf},
pmid = {33118013},
journal = {Molecular Biology and Evolution},
volume = {38},
number = {3},
pages = {1075--1089},
abstract = {Group II introns are large self-splicing RNA enzymes with a broad but somewhat irregular phylogenetic distribution. These ancient retromobile elements are the proposed ancestors of approximately half the human genome, including the abundant spliceosomal introns and non-LTR retrotransposons. In contrast to their eukaryotic derivatives, bacterial group II introns have largely been considered as harmful selfish mobile retroelements that parasitize the genome of their host. As a challenge to this view, we recently uncovered a new intergenic trans-splicing pathway that generates an assortment of mRNA chimeras. The ability of group II introns to combine disparate mRNA fragments was proposed to increase the genetic diversity of the bacterial host by shuffling coding sequences. Here we show that the Ll.LtrB and Ef.PcfG group II introns from Lactococcus lactis and Enterococcus faecalis respectively can both use the intergenic trans-splicing pathway to catalyze the formation of chimeric relaxase mRNAs and functional proteins. We demonstrated that some of these compound relaxase enzymes yield gain-of-function phenotypes, being significantly more efficient than their precursor wild-type enzymes at supporting bacterial conjugation. We also found that relaxase enzymes with shuffled functional domains are produced in biologically relevant settings under natural expression levels. Finally, we uncovered examples of lactococcal chimeric relaxase genes with junctions exactly at the intron insertion site. Overall, our work demonstrates that the genetic diversity generated by group II introns, at the RNA level by intergenic trans-splicing and at the DNA level most likely by recombination, can yield new functional enzymes with shuffled exons, which can lead to gain-of-function phenotypes. }
}
- Show RIS reference
TY - JOUR
ID - 31270
AU - LaRoche-Johnston,Felix
AU - Bosan,Rafia
AU - Cousineau,Benoit
T1 - Group II introns generate functional chimeric relaxase enzymes with modified specificities through exon shuffling at both the RNA and DNA level
PY - 2020
KW - Group II introns
KW - Lactococcus lactis
KW - Enterococcus faecalis
KW - Ll.LtrB
KW - Ef.PcfG
KW - conjugation
KW - trans-splicing
KW - bacterial genetics
KW - genetic diversity
KW - molecular evolution.
UR - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947834/pdf/msaa275.pdf
N2 - Group II introns are large self-splicing RNA enzymes with a broad but somewhat irregular phylogenetic distribution. These ancient retromobile elements are the proposed ancestors of approximately half the human genome, including the abundant spliceosomal introns and non-LTR retrotransposons. In contrast to their eukaryotic derivatives, bacterial group II introns have largely been considered as harmful selfish mobile retroelements that parasitize the genome of their host. As a challenge to this view, we recently uncovered a new intergenic trans-splicing pathway that generates an assortment of mRNA chimeras. The ability of group II introns to combine disparate mRNA fragments was proposed to increase the genetic diversity of the bacterial host by shuffling coding sequences. Here we show that the Ll.LtrB and Ef.PcfG group II introns from Lactococcus lactis and Enterococcus faecalis respectively can both use the intergenic trans-splicing pathway to catalyze the formation of chimeric relaxase mRNAs and functional proteins. We demonstrated that some of these compound relaxase enzymes yield gain-of-function phenotypes, being significantly more efficient than their precursor wild-type enzymes at supporting bacterial conjugation. We also found that relaxase enzymes with shuffled functional domains are produced in biologically relevant settings under natural expression levels. Finally, we uncovered examples of lactococcal chimeric relaxase genes with junctions exactly at the intron insertion site. Overall, our work demonstrates that the genetic diversity generated by group II introns, at the RNA level by intergenic trans-splicing and at the DNA level most likely by recombination, can yield new functional enzymes with shuffled exons, which can lead to gain-of-function phenotypes.
L3 - 10.1093/molbev/msaa275
JF - Molecular Biology and Evolution
VL - 38
IS - 3
SP - 1075
EP - 1089
ER -