@ARTICLE{TreeBASE2Ref31270,
author = {Felix LaRoche-Johnston and Rafia Bosan and Benoit Cousineau},
title = {Group II introns generate functional chimeric relaxase enzymes with modified specificities through exon shuffling at both the RNA and DNA level},
year = {2020},
keywords = {Group II introns, Lactococcus lactis, Enterococcus faecalis, Ll.LtrB, Ef.PcfG, conjugation, trans-splicing, bacterial genetics, genetic diversity, molecular evolution.},
doi = {10.1093/molbev/msaa275},
url = {http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947834/pdf/msaa275.pdf},
pmid = {33118013},
journal = {Molecular Biology and Evolution},
volume = {38},
number = {3},
pages = {1075--1089},
abstract = {Group II introns are large self-splicing RNA enzymes with a broad but somewhat irregular phylogenetic distribution. These ancient retromobile elements are the proposed ancestors of approximately half the human genome, including the abundant spliceosomal introns and non-LTR retrotransposons. In contrast to their eukaryotic derivatives, bacterial group II introns have largely been considered as harmful selfish mobile retroelements that parasitize the genome of their host. As a challenge to this view, we recently uncovered a new intergenic trans-splicing pathway that generates an assortment of mRNA chimeras. The ability of group II introns to combine disparate mRNA fragments was proposed to increase the genetic diversity of the bacterial host by shuffling coding sequences. Here we show that the Ll.LtrB and Ef.PcfG group II introns from Lactococcus lactis and Enterococcus faecalis respectively can both use the intergenic trans-splicing pathway to catalyze the formation of chimeric relaxase mRNAs and functional proteins. We demonstrated that some of these compound relaxase enzymes yield gain-of-function phenotypes, being significantly more efficient than their precursor wild-type enzymes at supporting bacterial conjugation. We also found that relaxase enzymes with shuffled functional domains are produced in biologically relevant settings under natural expression levels. Finally, we uncovered examples of lactococcal chimeric relaxase genes with junctions exactly at the intron insertion site. Overall, our work demonstrates that the genetic diversity generated by group II introns, at the RNA level by intergenic trans-splicing and at the DNA level most likely by recombination, can yield new functional enzymes with shuffled exons, which can lead to gain-of-function phenotypes. }
}
Taxa for Study 27000
Citation title:
"Group II introns generate functional chimeric relaxase enzymes with modified specificities through exon shuffling at both the RNA and DNA level".
Study name:
"Group II introns generate functional chimeric relaxase enzymes with modified specificities through exon shuffling at both the RNA and DNA level".
This study is part of submission 27000
(Status: Published).
Taxa
ID |
Taxon Label |
NCBI taxid |
uBIO namebankID |
4432291 |
Enterococcus faecalis pTEF4 |
1351
|
2555568
|
4432305 |
Lactococcus lactis 1064.C11 |
1358
|
229816
|
4432298 |
Lactococcus lactis 1064_C11 |
1358
|
229816
|
4432294 |
Lactococcus lactis 1AA59 |
1358
|
229816
|
4432290 |
Lactococcus lactis CF103 |
1358
|
229816
|
4432302 |
Lactococcus lactis CF112 |
1358
|
229816
|
4432299 |
Lactococcus lactis DmW198 |
1358
|
229816
|
4432303 |
Lactococcus lactis G50 |
1358
|
229816
|
4432296 |
Lactococcus lactis HP |
|
|
4432289 |
Lactococcus lactis KLDS |
|
|
4432288 |
Lactococcus lactis LMG9447 |
1358
|
229816
|
4432308 |
Lactococcus lactis p275D |
1358
|
229816
|
4432292 |
Lactococcus lactis pAF12 |
1358
|
229816
|
4432301 |
Lactococcus lactis pAH82 |
1358
|
229816
|
4432304 |
Lactococcus lactis pCV56A |
1358
|
229816
|
4432307 |
Lactococcus lactis pIL6 |
1358
|
229816
|
4432300 |
Lactococcus lactis pJM4A |
1358
|
229816
|
4432306 |
Lactococcus lactis pNZ712 |
1358
|
229816
|
4432287 |
Lactococcus lactis pRS01 |
1358
|
229816
|
4432293 |
Lactococcus lactis pSK11P |
1358
|
229816
|
4432295 |
Lactococcus lactis pUC063C |
1358
|
229816
|
4432286 |
Lactococcus lactis pUC77B |
1358
|
229816
|
4432297 |
Lactococcus raffinolactis Str_4877 |
1366
|
2556503
|