@ARTICLE{TreeBASE2Ref15428, author = {Katerina Fliegerov? and B. Hodrov? and Kerstin Voigt}, title = {Classical and Molecular Approaches as a Powerful Tool for the Characterization of Rumen Polycentric Fungi.}, year = {2004}, keywords = {}, doi = {}, url = {}, pmid = {}, journal = {Folia Microbiologica (Praha)}, volume = {49}, number = {2}, pages = {157--164}, abstract = {The ribosomal ITS 1 and ITS 2 fragments from 8 isolates of polycentric rumen anaerobic fungi were PCR-amplified and sequenced; the sequences obtained were aligned with published data and phylogenetic analyses were performed. The analysis of ITS 1 fragment clearly differentiated between the two polycentric genera Orpinomyces and Anaeromyces and these classification is supported by morphological observation. A multi-order phylogram based on ITS2 sequences proved that anaerobic rumen fungi are separated from aerobic chytrids, which form a well-supported monophylum with highest possible bootstrap proportion values of 100 % . Sequence analysis of ITS regions is a powerful tool for the classification of anaerobic fungi, however morphological description of strains is still necessary because some genera of rumen fungi display a high genetic heterogeneity.} }

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