TreeBASE Search-Trees for study 10445

@ARTICLE{TreeBASE2Ref18901, author = {Sita R Ghimire and Nikki Charlton and Jennifer Rudgers and Carolyn Young and Kelly D Craven}, title = {Prevalence of an intra-specific Neotyphodium hybrid in natural populations of Stout Wood Reed (Cinna arundinacea L.) from eastern North America}, year = {2010}, keywords = {Endophyte, ergot alkaloids, lolines, lolitrems, Neotyphodium schardlii, peramine, symbiosis}, doi = {}, url = {http://}, pmid = {}, journal = {Mycologia}, volume = {}, number = {}, pages = {}, abstract = {The members of genus Neotyphodium are asexual derivatives of sexual Epichlo? species and maintain endophytic relationships with many cool season grasses. Most Neotyphodium species analyzed so far are interspecific hybrids, with combined or partial genomes of two or three ancestors. In this study, we characterized Neotyphodium endophytes from Cinna arundinacea, a perennial cool season grass from eastern North America. A total of 23 isolates grouping into two distinct morphotypes were obtained from five local populations of C. arundinacea. PCR and cloning of translation-elongation factor 1-α (tefA) and beta-tubulin (tubB) genes of ten isolates comprising both morphotypes (two isolates location-1) revealed that all ten contain two copies of tef1 and tub2 genes. Surprisingly, phylogenetic analysis of mainly non-coding regions from these genes revealed that both copies of each isolate were inherited from Epichlo? typhina ancestors, suggesting the C. arundinacea endophytes arose through intra-specific hybridization between two E. typhina isolates from hosts Poa nemoralis and Poa pratensis. Profiling of alkaloid biosynthetic genes from these isolates suggests the presence of the peramine biosynthetic gene (perA) and the absence of genes required for biosynthesis of ergot alkaloid, lolines and lolitrems. Thus, this endophyte is potentially capable of producing peramine in planta and providing protection to its host from insect pests. The absence of genes for ergot and lolitrem alkaloid biosynthesis makes this endophyte a candidate for agricultural applications. Based on the phylogeny, alkaloid profiling and morphological characteristics, these isolates from C. arundinacea are now named as Neotyphodium schardlii, a new member of genus Neotyphodium and the first described to have arisen through an intraspecific hybridization event.} }

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Trees for Study 10445

Citation title: "Prevalence of an intra-specific Neotyphodium hybrid in natural populations of Stout Wood Reed (Cinna arundinacea L.) from eastern North America".

Study name: "Prevalence of an intra-specific Neotyphodium hybrid in natural populations of Stout Wood Reed (Cinna arundinacea L.) from eastern North America".

This study is part of submission 10435 (Status: Published).

Trees

ID	Tree Label	Tree Title	Tree Type	Tree Kind	Taxa
Tr8672	PAUP 4	Intra-specific Neotyphodium hybrid in Stout Wood Reed	Single	Species Tree	View Taxa
Tr8646	PAUP 2	Intra-specific Neotyphodium hybrid in Stout Wood Reed	Single	Species Tree	View Taxa
Tr8665	PAUP 21	Intra-specific Neotyphodium hybrid in Stout Wood Reed	Single	Species Tree	View Taxa
Tr8652	PAUP 8	Intra-specific Neotyphodium hybrid in Stout Wood Reed	Single	Species Tree	View Taxa
Tr8649	PAUP 5	Intra-specific Neotyphodium hybrid in Stout Wood Reed	Single	Species Tree	View Taxa
Tr8657	PAUP 13	Intra-specific Neotyphodium hybrid in Stout Wood Reed	Single	Species Tree	View Taxa
Tr8661	PAUP 17	Intra-specific Neotyphodium hybrid in Stout Wood Reed	Single	Species Tree	View Taxa
Tr8660	PAUP 16	Intra-specific Neotyphodium hybrid in Stout Wood Reed	Single	Species Tree	View Taxa
Tr8671	PAUP 3	Intra-specific Neotyphodium hybrid in Stout Wood Reed	Single	Species Tree	View Taxa
Tr8648	PAUP 4	Intra-specific Neotyphodium hybrid in Stout Wood Reed	Single	Species Tree	View Taxa
Tr8645	PAUP 1	Intra-specific Neotyphodium hybrid in Stout Wood Reed	Single	Species Tree	View Taxa
Tr8667	PAUP 23	Intra-specific Neotyphodium hybrid in Stout Wood Reed	Single	Species Tree	View Taxa
Tr8668	PAUP 24	Intra-specific Neotyphodium hybrid in Stout Wood Reed	Single	Species Tree	View Taxa
Tr8664	PAUP 20	Intra-specific Neotyphodium hybrid in Stout Wood Reed	Single	Species Tree	View Taxa
Tr8656	PAUP 12	Intra-specific Neotyphodium hybrid in Stout Wood Reed	Single	Species Tree	View Taxa
Tr8666	PAUP 22	Intra-specific Neotyphodium hybrid in Stout Wood Reed	Single	Species Tree	View Taxa
Tr8670	PAUP 2	Intra-specific Neotyphodium hybrid in Stout Wood Reed	Single	Species Tree	View Taxa
Tr8651	PAUP 7	Intra-specific Neotyphodium hybrid in Stout Wood Reed	Single	Species Tree	View Taxa
Tr8654	PAUP 10	Intra-specific Neotyphodium hybrid in Stout Wood Reed	Single	Species Tree	View Taxa
Tr8658	PAUP 14	Intra-specific Neotyphodium hybrid in Stout Wood Reed	Single	Species Tree	View Taxa
Tr8655	PAUP 11	Intra-specific Neotyphodium hybrid in Stout Wood Reed	Single	Species Tree	View Taxa
Tr8663	PAUP 19	Intra-specific Neotyphodium hybrid in Stout Wood Reed	Single	Species Tree	View Taxa
Tr8659	PAUP 15	Intra-specific Neotyphodium hybrid in Stout Wood Reed	Single	Species Tree	View Taxa
Tr8669	PAUP 1	Intra-specific Neotyphodium hybrid in Stout Wood Reed	Single	Species Tree	View Taxa
Tr8650	PAUP 6	Intra-specific Neotyphodium hybrid in Stout Wood Reed	Single	Species Tree	View Taxa
Tr8662	PAUP 18	Intra-specific Neotyphodium hybrid in Stout Wood Reed	Single	Species Tree	View Taxa
Tr8653	PAUP 9	Intra-specific Neotyphodium hybrid in Stout Wood Reed	Single	Species Tree	View Taxa
Tr8647	PAUP 3	Intra-specific Neotyphodium hybrid in Stout Wood Reed	Single	Species Tree	View Taxa