@ARTICLE{TreeBASE2Ref31676,
author = {Wu Zhang and Johannes (Ewald) Zacharias Groenewald and Lorenzo Lombard and Rene K. Schumacher and Alan John Lander Phillips and Pedro W. Crous},
title = {Evaluating species in Botryosphaeriales},
year = {2021},
keywords = {Multi-Locus Sequence Typing (MLST); canker and leaf spot pathogens; new taxa; systematics},
doi = {10.3767/persoonia.2021.46.03},
url = {http://doi.org/10.3767/persoonia.2021.46.03},
pmid = {},
journal = {Persoonia},
volume = {46},
number = {},
pages = {63--115},
abstract = {The Botryosphaeriales (Dothideomycetes) includes numerous endophytic, saprobic, and plant pathogenic species associated with a wide range of symptoms, most commonly on woody plants. In a recent phylogenetic treatment of 499 isolates in the culture collection (CBS) of the Westerdijk Institute, we evaluated the families and genera accommodated in this order of important fungi. The present study presents multigene phylogenetic analyses for an additional 230 isolates, using ITS, tef1, tub2, LSU and rpb2 loci, in combination with morphological data. Based on these data, 58 species are reduced to synonymy, and eight novel species are described. They include Diplodia afrocarpi (Afrocarpus, South Africa), Dothiorella diospyricola (Diospyros, South Africa), Lasiodiplodia acaciae (Acacia, Indonesia), Neofusicoccum podocarpi (Podocarpus, South Africa), N. rapaneae (Rapanea, South Africa), Phaeobotryon ulmi (Ulmus, Germany), Saccharata grevilleae (Grevillea, Australia) and S. hakeiphila (Hakea, Australia). The results have clarified the identity of numerous isolates that lacked Latin binomials or had been deposited under incorrect names in the CBS collection in the past. They also provide a solid foundation for more in-depth future studies on taxa in the order. Sequences of the tef1, tub2 and rpb2 genes proved to be the most reliable markers. At the species level, results showed that the most informative genes were inconsistent, but that a combination of four candidate barcodes (ITS, tef1, tub2 and rpb2) provided reliable resolution. Furthermore, given the large number of additional isolates included in this study, and newly generated multigene DNA datasets, several species could also be reduced to synonymy. The study illustrates the value of reassessing the identity of older collections in culture collections utilising modern taxonomic frameworks and methods.}
}
Matrices for Study 27596

Citation title:
"Evaluating species in Botryosphaeriales".

Study name:
"Evaluating species in Botryosphaeriales".

This study is part of submission 27596
(Status: Published).
Matrices
ID |
Matrix Title |
Description |
Data type |
NTAX |
NCHAR |
Taxa |
|
|
|
|
M57825
|
Fig. S2A Diplodia ITS alignment |
|
Nucleic Acid |
179 |
522 |
View Taxa
|
|
|
|
|
M57886
|
Fig. S8A Phaeobotryon ITS alignment |
|
Nucleic Acid |
54 |
500 |
View Taxa
|
|
|
|
|
M57876
|
Fig. S5D Neofusicoccum TUB2 alignment |
|
Nucleic Acid |
203 |
381 |
View Taxa
|
|
|
|
|
M57813
|
Fig. 2. Diplodia ITS TEF1 TUB2 alignment |
|
Nucleic Acid |
180 |
1291 |
View Taxa
|
|
|
|
|
M57896
|
Fig. S10C Saccharata TEF1 alignment |
|
Nucleic Acid |
39 |
302 |
View Taxa
|
|
|
|
|
M57821
|
Fig. 10 Saccharata ITS TEF1 RPB2 alignment |
|
Nucleic Acid |
41 |
1529 |
View Taxa
|
|
|
|
|
M57872
|
Fig. S5A Neofusicoccum ITS alignment |
|
Nucleic Acid |
208 |
510 |
View Taxa
|
|
|
|
|
M57864
|
Fig. S3B Dothiorella TEF1 alignment |
|
Nucleic Acid |
121 |
269 |
View Taxa
|
|
|
|
|
M57815
|
Fig. 4 Lasiodiplodia ITS TEF1 TUB2 RPB2 alignment |
|
Nucleic Acid |
148 |
1612 |
View Taxa
|
|
|
|
|
M57875
|
Fig. S5C Neofusicoccum TEF1 alignment |
|
Nucleic Acid |
200 |
275 |
View Taxa
|
|
|
|
|
M57885
|
Fig. S7C Neoscytalidium TUB2 alignment |
|
Nucleic Acid |
65 |
358 |
View Taxa
|
|
|
|
|
M57889
|
Fig. S9A Pseudofusicoccum ITS alignment |
|
Nucleic Acid |
30 |
493 |
View Taxa
|
|
|
|
|
M57820
|
Fig. 9 Pseudofusicoccum TEF1 TUB2 ITS alignment |
|
Nucleic Acid |
30 |
1256 |
View Taxa
|
|
|
|
|
M57877
|
Fig. S6A Neofusicoccum parvum complex ITS alignment |
|
Nucleic Acid |
75 |
481 |
View Taxa
|
|
|
|
|
M57880
|
Fig. S6C Neofusicoccum parvum complex TEF1 alignment |
|
Nucleic Acid |
71 |
281 |
View Taxa
|
|
|
|
|
M57890
|
Fig. S9B Pseudofusicoccum TEF1 alignment |
|
Nucleic Acid |
30 |
295 |
View Taxa
|
|
|
|
|
M57883
|
Fig. S7A Neoscytalidium ITS alignment |
|
Nucleic Acid |
66 |
499 |
View Taxa
|
|
|
|
|
M57892
|
Fig. S10A Saccharata ITS alignment |
|
Nucleic Acid |
41 |
550 |
View Taxa
|
|
|
|
|
M57869
|
Fig. S4C Lasiodiplodia TEF1 alignment |
|
Nucleic Acid |
146 |
258 |
View Taxa
|
|
|
|
|
M57817
|
Fig. 6 Neofusicoccum parvum ITS TEF1 TUB2 RPB2 alignment |
|
Nucleic Acid |
75 |
1805 |
View Taxa
|
|
|
|
|
M57879
|
Fig. S6B Neofusicoccum parvum complex RPB2 alignment |
|
Nucleic Acid |
61 |
535 |
View Taxa
|
|
|
|
|
M57884
|
Fig. S7B Neoscytalidium TEF1 alignment |
|
Nucleic Acid |
65 |
187 |
View Taxa
|
|
|
|
|
M57862
|
Fig. S2C Diplodia TUB2 alignment |
|
Nucleic Acid |
167 |
389 |
View Taxa
|
|
|
|
|
M57812
|
Fig. 1. Botryosphaeria ITS TEF1 TUB2 alignment |
|
Nucleic Acid |
51 |
1206 |
View Taxa
|
|
|
|
|
M57891
|
Fig. S9C Pseudofusicoccum TUB2 alignment |
|
Nucleic Acid |
23 |
427 |
View Taxa
|
|
|
|
|
M57822
|
Fig. S1A Botryosphaeria ITS alignment |
|
Nucleic Acid |
51 |
461 |
View Taxa
|
|
|
|
|
M57888
|
Fig. S8C Phaeobotryon TEF1 alignment |
|
Nucleic Acid |
45 |
339 |
View Taxa
|
|
|
|
|
M57868
|
Fig. S4B Lasiodiplodia RPB2 alignment |
|
Nucleic Acid |
112 |
520 |
View Taxa
|
|
|
|
|
M57887
|
Fig. S8B Phaeobotryon LSU alignment |
|
Nucleic Acid |
44 |
556 |
View Taxa
|
|
|
|
|
M57819
|
Fig. 8 Phaeobotryon ITS LSU TEF1 alignment |
|
Nucleic Acid |
54 |
1726 |
View Taxa
|
|
|
|
|
M57824
|
Fig. S1C Botryosphaeria TUB2 alignment |
|
Nucleic Acid |
44 |
387 |
View Taxa
|
|
|
|
|
M57871
|
Fig. S4D Lasiodiplodia TUB2 alignment |
|
Nucleic Acid |
130 |
402 |
View Taxa
|
|
|
|
|
M57816
|
Fig. 5 Neofusicoccum ITS TEF1 TUB2 RPB2 alignment |
|
Nucleic Acid |
208 |
1840 |
View Taxa
|
|
|
|
|
M57818
|
Fig. 7 Neoscytalidium ITS TEF1 TUB2 alignment |
|
Nucleic Acid |
66 |
1133 |
View Taxa
|
|
|
|
|
M57865
|
Fig. S3C Dothiorella TUB2 alignment |
|
Nucleic Acid |
109 |
415 |
View Taxa
|
|
|
|
|
M57823
|
Fig. S1B Botryosphaeria TEF1 alignment |
|
Nucleic Acid |
50 |
263 |
View Taxa
|
|
|
|
|
M57881
|
Fig. S6D Neofusicoccum parvum complex TUB2 alignment |
|
Nucleic Acid |
72 |
394 |
View Taxa
|
|
|
|
|
M57893
|
Fig. S10B Saccharata RPB2 alignment |
|
Nucleic Acid |
32 |
627 |
View Taxa
|
|
|
|
|
M57866
|
Fig. S4A Lasiodiplodia ITS alignment |
|
Nucleic Acid |
147 |
368 |
View Taxa
|
|
|
|
|
M57826
|
Fig. S2B Diplodia TEF1 alignment |
|
Nucleic Acid |
174 |
276 |
View Taxa
|
|
|
|
|
M57874
|
Fig. S5B Neofusicoccum RPB2 alignment |
|
Nucleic Acid |
176 |
539 |
View Taxa
|
|
|
|
|
M57863
|
Fig. S3A Dothiorella ITS alignment |
|
Nucleic Acid |
123 |
479 |
View Taxa
|
|
|
|
|
M57814
|
Fig. 3 Dothiorella ITS TEF1 TUB2 alignment |
|
Nucleic Acid |
123 |
1272 |
View Taxa
|
|
|
|
|